CombiSEAL: New Pathfinder for New Protein Discovery
- Field
- Research Tools and Methods
- Reference No.
- IP00803
Key Problem and Market Opportunity
- Using site-directed mutagenesis for protein engineering to make better enzymes, antibodies, and protein drugs lacks throughput and scalability
- Protein function not only depends on one or two amino acids but a combination of changes in many cases
Key Advantages of the Technology
- Simultaneously study the interaction of different amino acids in a protein at a much faster speed
- First platform for assembling and barcoding protein-encoding sequences carrying multiple mutations en masse
- Coupled with next-generation sequencing to track all variants in an unprecedented throughput
- Uses barcoded sequences to easily identify the functional variants
- Helped to identify a better error-free Cas9 protein
Further Details
Choi GC, et al. Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Nat Methods 16, 722–730 (2019).
Potential Product and Services
- Drug discovery service
- Reagent kits
- Automatic platform to the combinatorial constructs
Development Status
Patents
- PCT Application No.: PCT/CN2019/106096
IP Status
- Patent application submitted