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Bistriflate-based Fluorogenic Probe for Detection of Superoxide

Bistriflate-based Fluorogenic Probe for Detection of Superoxide
Research Tools and Methods

Background and Market Opportunity

  • Superoxide, reactive oxygen or nitrogen species are indicators of pathophysiological processes associated with aging, inflammation and progression of several diseases such ascancer and diabetics.
  • Analytical techniques such as chemiluminescence and fluorescence have been developed to detect the intracellular generation of reactive species, especially the generation of superoxide in mitochondria.
  • Further, probes such as MitoSOX and Dihydroethidium (HE) are available in the market to detect the intracellular and mitochondria superoxide.
  • However, these techniques and probes have poor selectivity and sensitivity.

Technology Overview and Key Advantages

Technology Section Slider 1
  • The current invention discloses series of fluorescent probes that exhibit excellent sensitivity and selectivity for superoxide over a wide range of oxidants and reductants in the mitochondria of living cells.
  • The probes give different fluorescent colors such as green, yellow, red or far-red.  The performance of the probes was robust in multiple cellular models including inflammation and mitochondrial inhibition
  • Experimental data indicted superiority of our technology against MitoSOX as highlighted in Table 1.


  • Can differentiate between potent stimulants from strong to weak, hence have high sensitivity.
  • Exhibit very strong signal towards superoxide and therefore exhibits high selectivity.
  • Non-toxic.

Potential Product and Applications

  • A tool to study the oxidative stress in various pathologies.
  • Probes for drug screening.

Development Status and IP Strength

  • National phase patent application filed in the USA, China and Europe.

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